Signalling
Part:BBa_K3425108:Design
Designed by: Rubén Crespo Blanco Group: iGEM20_UofUppsala (2020-10-23)
pCadBA testing construct (pSB1K01)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2179
Illegal PstI site found at 12 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2179
Illegal PstI site found at 12 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2179
Illegal XhoI site found at 1029
Illegal XhoI site found at 2055 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2179
Illegal PstI site found at 12 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2179
Illegal PstI site found at 12
Illegal AgeI site found at 2912
Illegal AgeI site found at 3024 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2185
Illegal SapI.rc site found at 5
Design Notes
Copy number seems to affect greatly the level of expression attainable by this part. Also, it's important to not have excess expression of BBa_K3425100 to prevent leaky expression. Also, remember that BBa_K3425101 alone does not have an RBS!
Source
Same as BBa_K3425101.